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. 2009 Feb 25;37(7):2336–2345. doi: 10.1093/nar/gkp096

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — UvsY effects on ssDNA-dependent ATPase activities of UvsX, UvsX-H195A and UvsX-H195Q. Reaction velocities were measured by coupled spectrophotometric assay as described in Materials and Methods section. Reactions contained 0.4 µM UvsX (closed circles), UvsX-H195A (closed squares) or UvsX-H195Q (closed diamonds). ssDNA concentration was 4.5 µM and ATP concentration was 2 mM in all reactions, and UvsY concentration varied as indicated. All other conditions were as described in Materials and Methods section.