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. 2009 Feb 25;37(7):2336–2345. doi: 10.1093/nar/gkp096

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Effects of wild-type and mutant UvsY proteins on ADP and AMP production by UvsX ssDNA-dependent ATPase activity. Velocities of ADP and AMP production were measured by TLC assay as described in Materials and Methods section. All reactions contained 0.45 µM UvsX, 4.5 µM ssDNA and 4 mM α-[³²P]-ATP. All other conditions were as described in Materials and Methods section. (A) Velocity of ADP production by wild-type UvsX protein as a function of UvsY (closed circles), UvsY-SM (closed squares) or UvsY-DM (closed diamonds) concentration. (B) Velocity of AMP production by wild-type UvsX protein as a function of UvsY (closed circles), UvsY-SM (closed squares) or UvsY-DM (closed diamonds) concentration. Note that (B) is plotted on an expanded scale compared to (A). (C) ADP/AMP product ratio for wild-type UvsX protein as a function of UvsY (closed circles), UvsY-SM (closed squares) or UvsY-DM (closed diamonds) concentration.