Figure 3.

Effects of wild-type and mutant UvsY proteins on ADP production by (A) UvsX-H195A mutant and (B) UvsX-H195Q mutant ssDNA-dependent ATPase activities. Velocities of ADP production were measured by TLC assay as described in Materials and Methods section. All reactions contained 0.45 µM recombinase, 4.5 µM ssDNA, 4 mM α-[³²P]-ATP and variable concentrations of either UvsY (closed circles), UvsY-SM (closed squares) or UvsY-DM (closed diamonds). All other conditions were as described in Materials and Methods section.