Table 2.
Morphological, Immunophenotypic and Molecular Characteristics
| Case No. |
Morphology |
Immunohistochemistry |
Molecular Biology |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WHO Grade |
Prominent IF Areas |
LELs | DLBCL (%Area) |
Presence of Lymphocytic Thyroiditis |
CD10 |
Bcl-6 |
Bcl-2 |
IGH-BCL2 |
BCL6 translocation |
|||
| FCCs | IF Cells | FCCs | IF Cells | (FCCs) | ||||||||
| 1 | 3b | N | Present | N | Y | - | - | + | - (↓) | - | - | - |
| 2 | 3a | Y | Prominent | Y (50%) | Y | - | - | + | - (↓) | - | - | - |
| 3 | 3a | Y | Prominent | N | N | - | - | + | - (↓) | - | - | nd |
| 4 | 3a | N | Prominent | N | N | - | - | + | Weak (↓) | - | - | nd |
| 5 | 3a | Y | Present | Y (40%) | Y | + | - (↓) | + | Strong | - | - | nd |
| 6 | 3a | Y | Present | N | N | + | - (↓) | + | Weak (↓) | - | - | - |
| 7 | 2 | Y | Present | N | N | + | Weak (↓) | + | Strong | - | - | - |
| 8 | 1 | Y | Prominent | N | Y | + | - (↓) | + | - (↓) | - | - | - (3 signals) |
| 9 | 2 | N | Present | N | Y | + | - (↓) | Failed | Failed | - | nd | nd |
| 10 | 3a | Y | Prominent | Y (<10%) | N | + | Weak (↓) | + | Weak (↓) | Weak | - | + |
| 11 | 2 | Y | Prominent | N | N | + | - (↓) | + | - (↓) | Weak | - | - |
| 12 | 1 | N | Present | N | Y | + | Strong | + | Weak (↓) | - | + | - |
| 13 | 1 | N | Prominent | N | Y | - | - | + | Weak (↓) | + | + | + |
| 14 | 3a | N | Present | Y (80%) | Y | + | Strong | + | Weak (↓) | + | + | - (3 signals) |
| 15 | 2 | Y | Prominent | N | Y | + | Weak (↓) | + | Strong | + | + | - |
| 16 | 2 | Y | Prominent | N | N | + | Weak (↓) | + | Weak (↓) | + | + | - (3 signals) |
| 17 | 2 | Y | Present | N | N | + | Weak (↓) | + | - (↓) | + | + | nd |
| 18 | 1 | Y | Prominent | N | N | + | Strong | + | Strong | + | + | nd |
| 19 | 1 | Y | Prominent | N | N | + | Weak (↓) | + | - (↓) | + | + | nd |
| 20 | 2 | N | Prominent | Y (80%) | N | + | Strong | + | Strong | + | nd | nd |
| 21 | 2 | Y | Present | Y | N | + | Weak (↓) | + | - (↓) | + | + | nd |
| 22 | 2 | Y | Present | N | Y | + | Strong | + | Weak (↓) | + | + | nd |
FCCs, follicle centre cells; IF, interfollicular; IgH, immunoglobulin heavy chain; nd, not done; (↓) staining in IF cells reduced compared to FCC; IGH-BCL2 translocation status was examined by FISH & PCR (cases 1, 2, 6, 8, 10-13, 15, & 16), FISH (cases 7, 14, & 21), or PCR (cases 3-5, 18, 19, & 22).