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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Cell Metab. 2009 Mar;9(3):287–302. doi: 10.1016/j.cmet.2009.01.013

Figure 1.

Figure 1

(A) Regulation of Sox9 expression by Pref-1. Coomassie staining of purified Pref-1-hFc (a). RT-PCR (b), RT-qPCR (c) and Western blotting (d) in Pref-1 null MEFs treated with 50 nM Pref-1-hFc or 50 ng/ml TNFα. Results are mean ± SEM; **, P<0.01 compared to that before treatment. (B) Western blotting of cells with Pref-1-hFc treatment (a), ERK1/2 inhibitor (b), and ERK1/2 siRNA transfection (c). (C) RT-PCR for adipose tissue (Ad tissue), stromal vascular fraction (SVF) and adipocyte fraction (Ad F). (D) Pref-1 regulates Sox9 expression during adipocyte differentiation. (E) Comparison of adipocyte differentiation of MEFs from Pref-1 null, wild-type and aP2-Pref-1 transgenic embryos. Northern blot (left panel), RT-qPCR, and Western blot analysis (middle panels) in MEFs at confluence. The value for wild-type MEFs was defined as 1. Results are mean ± SEM; **, P < 0.01 compared to wild-type MEFs. Microscopic morphology, Oil red O staining (right upper panels) and adipogenic transcription factors by Northern blotting and late adipocyte markers by RT-PCR 8 days after induction of adipocyte differentiation (right lower panels).

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