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. Author manuscript; available in PMC: 2010 Mar 1.

Published in final edited form as: Cell Metab. 2009 Mar;9(3):287–302. doi: 10.1016/j.cmet.2009.01.013

Pref-1 inhibits 3T3-L1 adipocyte differentiation through Sox9. (A) RT-PCR (a) and RT-qPCR (b and c) for various adipocyte markers during 3T3-L1 adipocyte differentiation. RT-qPCR after 12 h treatment (d). (B) Sox9 protein level after 48 h of infection (left panel). Morphology, Oil red O staining (middle panel), and RT-qPCR and RT-PCR for adipocyte markers (right panels). (C) Sox9 knockdown enhances adipocyte differentiation. Western blotting after 48 h siRNA transfection (left panel). Morphology, Oil red O staining and adipocyte marker expression after adipocyte differentiation. (D) 3T3-F442A-GFP preadipocytes stably overexpressing or with knockdown of Sox9, or control cells (3×10⁷ cells mixed with Matrigel matrix (BD) per site) were injected subcutaneously into the back of SCID mice. The resulting tissues were dissected for Oil red O and DAPI staining and RT-qPCR (n = 4/group).

Pref-1 inhibits 3T3-L1 adipocyte differentiation through Sox9. (A) RT-PCR (a) and RT-qPCR (b and c) for various adipocyte markers during 3T3-L1 adipocyte differentiation. RT-qPCR after 12 h treatment (d). (B) Sox9 protein level after 48 h of infection (left panel). Morphology, Oil red O staining (middle panel), and RT-qPCR and RT-PCR for adipocyte markers (right panels). (C) Sox9 knockdown enhances adipocyte differentiation. Western blotting after 48 h siRNA transfection (left panel). Morphology, Oil red O staining and adipocyte marker expression after adipocyte differentiation. (D) 3T3-F442A-GFP preadipocytes stably overexpressing or with knockdown of Sox9, or control cells (3×10⁷ cells mixed with Matrigel matrix (BD) per site) were injected subcutaneously into the back of SCID mice. The resulting tissues were dissected for Oil red O and DAPI staining and RT-qPCR (n = 4/group).

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