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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Cell Metab. 2009 Mar;9(3):287–302. doi: 10.1016/j.cmet.2009.01.013

Figure 6.

Figure 6

Pref-1 directs MEF commitment to chondrogenic lineage through Sox9 regulation. (A) Micromass culture of MEFs. Alcian blue staining (left panel) and RT-PCR for chondrogenic markers (right panel) after 14 days of culture. (B) Pref-1 null MEFs infected with Sox9 lentivirus after 10 days micromass culture. **, P<0.01 compared to differentiated cells infected with control empty lentivirus and in the presence of control hFc. (C) Knockdown of Sox9 blocks chondrogenic induction by Pref-1. Alcian blue staining for Pref-1 null MEFs transfected with siRNA after micromass culture. Western blotting after 48 h transfection (left top panel). Results are mean ± SEM; *, P<0.05 and **, P<0.01 compared to cells transfected with control siRNA and treated with hFc.

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