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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Methods. 2009 Feb 20;47(4):283–290. doi: 10.1016/j.ymeth.2009.02.005

Fig. 3.

Fig. 3

Southern blot assay for PIC function. Integration of 9.7 kb HIV-1 cDNA into linearized 5.4 kb φX174 yields a 15.1 kb DNA recombination intermediate. Reaction mixtures following deproteinization are fractionated by agarose gel electrophoresis. After Southern blotting with a virus-specific probe, DNA strand transfer activity is defined as the percent of cDNA substrate converted into reaction product (lane 2; φX174 DNA was omitted from the integration reaction analyzed in lane 1). Though integration occurs essentially throughout the φX174 genome, the resulting population of recombinants displays an electrophoretic mobility consistent with linear double-stranded 15.1 kb DNA.