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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Methods. 2009 Feb 20;47(4):283–290. doi: 10.1016/j.ymeth.2009.02.005

Fig. 4.

Fig. 4

Partial PIC purification by spin column chromatography. (A) To accommodate the Bio-Rad Econo-Pac column in an appropriate centrifuge, it may be necessary to severe off the top as indicated. An approximate 0.5 ml section of a 5 ml serological pipet is used to prop the column up from the bottom of a 50 ml conical tube during centrifugation. (B) The spun bed should appear as a contracted cone with flattened top.