Table 1.

Top Affected Gene Functions in Fed or Starved dHNF4 Mutants Reveal a Central Role in Fatty Acid β-Oxidation

Fed Animals
GO category	# genes (total)	p-value
oxidoreductase activity	17 (541)	4.8e-07
carnitine o-acyl transferase activity	4 (6)	2.3e-06
mitochondrion	15 (521)	6.2e-06
transferase activity, other than amino-acyl groups	7 (75)	1.0e-05
acyltransferase activity	7 (75)	1.0e-05
cytoplasmic part	22 (1537)	1.8e-05
lipid particle	8 (129)	1.9e-05
transferase activity, transferring acyl groups	7 (88)	1.9e-05
fatty acid metabolic process	5 (35)	4.9e-05
fatty acid β-oxidation	3 (5)	6.3e-05
fatty acid oxidation	3 (6)	1.1e-04
o-acyltransferase activity	4 (21)	1.4e-04
Starved Animals
GO category	# genes (total)	p-value
oxidoreductase activity	29 (541)	3.9e-14
fatty acid metabolic process	10 (35)	3.4e-09
cellular lipid metabolic process	15 (113)	3.4e-09
lipid metabolic process	15 (125)	1.1e-08
fatty acid β-oxidation	5 (5)	4.9e-08
monocarboxylic acid metabolic process	10 (53)	1.4e-07
fatty acid oxidation	5 (6)	2.1e-07
transferase activity, other than amino-acyl groups	11 (75)	2.3e-07
acyltransferase activity	11 (75)	2.3e-07
hydrolase activity	44 (1550)	9.5e-07
transferase activity, transferring acyl groups	11 (88)	1.1e-06
γ-butyrobetaine dioxygenase activity	4 (4)	1.6e-06

GOstat analysis of either the 74 genes that are down-regulated in fed dHNF4 mutants compared to fed control larvae (Supplemental Table 3) or the 197 genes that are down-regulated in starved dHNF4 mutants compared to starved control larvae (Supplemental Table 5). The top twelve GO categories are listed, from top to bottom, along with the number of affected genes in that category, the total number of genes in that category in parentheses, and the p-value of the match (http://gostat.wehi.edu.au/cgi-bin/goStat.pl).