Figure 7.

H&E and immunohistochemical staining of rat hind paw tissue from rats with and without fracture and positive control rats with hindpaw incision. The dorsal hind paw skin from rats 4 weeks after fracture, 3 and 7 days after incision were harvested for these studies. In the top row are representative H&E stained sections. Cellular infiltration was observed only in skin from rats incised 3 days earlier. The top row for fluorescent microscopy contains micrographs from sections exposed to antibodies for OX-42, a macrophage marker. No specific staining was noted in the fractured hind paw skin, while a robust response is observed in the skin incised 3 days earlier. The middle row for fluorescent microscopy shows sections exposed to antibodies for CD-43, a T-lymphocyte marker. Again, no specific staining was noted in the fracture group, though abundant T-lymphocytes were identified in skin from rats incised 7 days earlier. The bottom row shows no specific staining observed in the fracture hindpaw for HIS48, a neutrophil marker, but robust staining in the rats incised 3 days earlier. Scale bar: top panel - 100μm, middle and bottom panels -25μm.