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. Author manuscript; available in PMC: 2010 Feb 3.
Published in final edited form as: Cancer Cell. 2009 Feb 3;15(2):114–123. doi: 10.1016/j.ccr.2008.12.018

Figure 1. IL-23 and IL-12 expression in tumor-infiltrating myeloid cells is compartmentalized and oppositely regulated by Stat3.

Figure 1

ELISA measurement of protein levels of IL-23 (A) and IL-12 (B) in supernatants of tumor cell lines (B16 and C4 melanoma cells), B16 whole tumor cell suspensions (Total tumor) from mice with Stat3+/+ or Stat3−/− hematopoietic cells, enriched tumor-infiltrating CD11b+CD11c− macrophages (MACs) and CD11c+ dendritic cells (DCs) cultured in vitro for 24 h. Data shown are mean values ± SD from one of three separate experiments performed on cells pooled from four animals per group analyzed. (C) Western blot detection of IL-23/p19 protein levels in the CD11b+ cells isolated from the whole B16 tumor and tumor-draining lymph nodes of Stat3+/+ or Stat3−/− mice. (D, E) IL-23 and IL-12 protein levels measured by ELISA as in Fig. 1A, in supernatants from the enriched tumor-infiltrating CD11b+CD11c− MACs and CD11c+ DCs isolated from the whole MC38 colon carcinoma (D) and MB49 bladder carcinoma (E) tumors; mean ± SEM (n = 3).