Figure 6.

DOC2B overexpression does not change the amount or mobility of vesicles in the submembrane region of chromaffin cells. A–C, Comparison of NPY-Venus-containing vesicle density and mobility by TIRFM in DOC2B^wt (white; 189 vesicles from 25 cells), DOC2B^D218,220N (gray; 158 vesicles from 23 cells), and EGFP control (black; 137 vesicles from 20 cells). A, Vesicle density per μm² surface area of chromaffin cell footprint. B, C, Individual vesicles were analyzed for mobility. B, Mobility of vesicles in the x–y directions, measured as the MSD relative to the start of the experiment. C, Caging diameter, defined as the maximal distance between two points in the x–y directions in 6 s. Open triangles indicate vesicles from fixed cells as a control in mobility analysis (158 vesicles from 19 cells). Data represent means ± SEM.