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The C2B domain of DOC2B interacts with the syntaxin/SNAP-25 dimer. GST-C2B, attached to glutathione beads, binds syntaxin/SNAP-25 heterodimer, but not syntaxin or SNAP-25 alone. A, Visualization using Sypro Ruby protein stain. The asterisk denotes a GST breakdown product associated with the GST-C2B beads. B, Western immunoblotting with an anti-SNAP-25 antibody confirms that SNAP-25 binds DOC2B only in tandem with syntaxin.
