Table 3.
Effects of hypobaric hypoxia on mitochondrial respiration
| Normoxic | Hypoxic | |
|---|---|---|
| RV mitochondrial function | ||
| State 3/mg protein (nmol O2/min/mg protein) | 57.4 ± 8.3 | 65.3 ± 20.3 |
| ADP/O ratio | 2.02 ± 0.2 | 2.7 ± 0.1** |
| Rate of ADP phosphorylation (nmol ADP/min/mg protein) | 122 ± 20.2 | 125 ± 32.1 |
| State 4 respiration (nmol O2/min/mg protein) | 3.0 ± 0.7 | 4.3 ± 0.9 |
| Basal leak (nmol O2/min/mg protein) | 12.1 ± 1.5 | 2.6 ± 0.6** |
| RCI (State 3/State 4) | 14.7 ± 4.3 | 15.3 ± 6.5 |
| LV mitochondrial function | ||
| State 3/mg protein (nmol O2/min/mg protein) | 118 ± 11.6 | 75.8 ± 10.5* |
| ADP/O ratio | 2.6 ± 0.2 | 2.5 ± 0.2 |
| Rate of ADP phosphorylation (nmol ADP/min/mg protein) | 231 ± 17.8 | 186 ± 22.9 |
| State 4 respiration (nmol O2/min/mg protein) | 6.6 ± 1.6 | 3.9 ± 0.8 |
| Basal leak (nmol O2/min/mg protein) | 8.1 ± 1.9 | 17.8 ± 4.8* |
| RCI (State 3/State 4) | 39.1 ± 10 | 12.7 ± 2.6* |
Rats were exposed to hypobaric hypoxia (11% O2) for 4 weeks and compared with age-matched normoxic groups. Data are represented as means ± SEM.
*
P < 0.05 and
**
P < 0.01 vs. normoxic controls (n = 6 animals per group)