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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Comp Neurol. 2009 Jan 1;512(1):27–51. doi: 10.1002/cne.21906

Figure 2.

Figure 2

High magnification photomicrographs of Nissl-stained neurons in the monkey hippocampal formation. A-C: granule cells of the dentate gyrus. A: PM-15-03, perfusion-fixed; B: PM-10-02, immersion-fixed 2 hours postmortem; C: PM-04-02, immersion-fixed 48 hours postmortem. Note the decreased soma size and increased staining intensity of dentate granule cells in immersion-fixed tissue. Scale bar in A: 20 μm, applies to panels A, B, C. D-F: pyramidal neurons in the CA1 field of the hippocampus. D: PM-15-03, perfusion-fixed; E: PM-03-02, immersion-fixed 2 hours postmortem; F: PM-02-02, immersion-fixed 48 hours postmortem. Note the decreased soma size and increased dendritic labeling of CA1 pyramidal neurons in immersion-fixed tissue. Scale bar in D: 50 μm, applies to panels D, E, F.