. Author manuscript; available in PMC: 2010 Jan 1.

Published in final edited form as: J Comp Neurol. 2009 Jan 1;512(1):27–51. doi: 10.1002/cne.21906

Table 2.

Summary of immunohistochemical procedures used in the study

	Primary antibody	Secondary antibody	Blocking serum	Buffer / Visualization
SMI-32	Monoclonal SMI-32 Sternberger, cat # SMI-32	Horse anti-mouse IgG Vector; cat# BA-2000	NHS, Biogenesis cat # 8270-1004	Tris Avidin-biotin
AChE Immuno	Monocolonal anti-AChe Abcam, cat # ab2803-200	Horse anti-mouse IgG Vector; cat# BA-2000	NHS, Biogenesis cat # 8270-1004	KPBS Avidin-biotin
Serotonin	Polyclonal anti-5-HT Diasorin, cat # 20080	Goat anti-rabbit IgG Vector; cat # AI-1000	NGS, Biogenesis cat # 8270-1204	PBS PAP
Parvalbumin	Monoclonal anti-parvalbumin SWANT, cat # 235	Horse anti-mouse IgG Vector; cat# BA-2000	NHS, Biogenesis cat # 8270-1004	KPBS Avidin-biotin
Calbindin-D28K	Monoclonal anti-calbindin SWANT, cat # 300	Horse anti-mouse IgG Vector; cat# BA-2000	NHS, Biogenesis cat # 8270-1004	KPBS Avidin-biotin
Calretinin	Monoclonal anti-calretinin SWANT, cat # 6B3	Horse anti-mouse IgG Vector; cat# BA-2000	NHS, Biogenesis cat # 8270-1004	KPBS Avidin-biotin
Somatostatin	Polyclonal anti-SS28_1-12 Gift of Dr. Robert Benoit	Goat anti-rabbit IgG Vector; cat # AI-1000	NGS, Biogenesis cat # 8270-1204	KPBS Avidin-biotin