Table 2.
Mitochondrial Free Matrix Calcium in Mature and Aged Male and Female Hearts
[Ca2+]Mito |
Probability |
||||||||
---|---|---|---|---|---|---|---|---|---|
Mature Male | Mature Female | Aged Male | Aged Female | Age | Gender | Age × Gender | Mature Male vs Female | Aged Male vs Female | |
Control | 217.0 (6.5) | 209.5 (7.2) | 202.7 (20.9) | 204.3 (18.5) | 0.6055 | 0.9511 | 0.8629 | 0.7257 | 0.9535 |
GI | 619.3 (16.5) | 593.2 (17.6) | 773.2 (17.9) | 762.7 (19.4) | <0.0001 | 0.3178 | 0.6662 | 0.3039 | 0.6993 |
K/Mg | 608.7 (13.8) | 602.5 (15.5) | 652.3 (19.1) | 668.0 (17.8) | 0.0039 | 0.7793 | 0.5213 | 0.7730 | 0.5630 |
K/Mg + DZX | 483.5 (19.5) | 572.7 (22.6) | 572.3 (16.9) | 665.0 (15.5) | 0.004 | 0.004 | 0.9724 | 0.0122 | 0.0127 |
Mature and aged, male and female mitochondrial free matrix calcium (nM/mg mitochondrial protein/mg dry weight) for Control (after 75 minutes of perfusion), GI, K/Mg and K/Mg + DZX (after 30 minutes of equilibrium, 30 minutes of ischemia. 15 minutes of reperfusion). All results are shown as mean (SEM) for n = 6 animals for each group. P values for specific comparisons between male and female animals, within an age group were calculated using a t test. Significant differences at p < 0.05 are shown in bold face.
DZX = diazoxide; GI = global ischemia; K/Mg = potassium/magnesium.