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. Author manuscript; available in PMC: 2009 Apr 27.
Published in final edited form as: Ann Thorac Surg. 2007 Mar;83(3):1102–1109. doi: 10.1016/j.athoracsur.2006.10.059

Table 3.

Malate Induced, Complex I and Succinate Induced, Complex II State 3, Active, Mitochondrial Oxygen Consumption in Mature and Aged Male and Female Hearts

Malate Induced Complex I
 Probability
Group
 Effects
 Comparisons
Mature Male Mature Female Aged Male Aged Female Age Gender Age × Gender Mature Male vs Female Aged Male vs Female
Control 152.1 ± 5.61 (44) 180.0 ± 5.5 (48) 101.3 ± 2.2 (47) 148.7 ± 3.3 (31) <0.0001 <0.0001 0.0388 0.0006 <0.0001
GI 119.7 ± 2.6 (55) 146.8 ± 5.3 (44) 89.9 ± 2.9 (46) 113.0 ± 3.6 (34) <0.0001 <0.0001 0.6026 <0.0001 <0.0001
K/Mg 136.3 ± 1.9 (61) 171.5 ± 4.0 (45) 93.8 ± 2.4 (48) 111.6 ± 5.2 (38) <0.0001 <0.0001 0.0083 <0.0001 0.0033
K/Mg + DZX 117.8 ± 2.2 (57) 164.4 ± 4.3 (44) 94.0 ± 2.1 (46) 105.6 ± 4.9 (40) <0.0001 <0.0001 <0.0001 <0.0001 0.0344
Succinate Induced Complex II
 Probability
Group
 Effects
 Comparisons
Mature Male Mature Female Aged Male Aged Female Age Gender Age × Gender Male vs Female Male vs Female
Control 176.9 ± 4.3 (39) 238.5 ± 5.8 (47) 158.6 ± 2.2 (50) 195.2 ± 3.8 (29) <0.0001 <0.0001 0.0053 <0.0001 <0.0001
GI 159.4 ± 1.8 (32) 181.7 ± 5.0 (38) 143.3 ± 2.9 (48) 168.9 ± 4.1 (32) <0.0001 <0.0001 0.6886 <0.0001 <0.0001
K/Mg 165.8 ± 2.4 (32) 218.7 ± 5.2 (48) 151.4 ± 2.9 (48) 180.3 ± 4.7 (32) <0.0001 <0.0001 0.0048 <0.0001 <0.0001
K/Mg + DZX 156.3 ± 1.8 (40) 204.1 ± 3.6 (48) 151.0 ± 1.5 (48) 164.9 ± 5.0 (32) <0.0001 <0.0001 <0.0001 <0.0001 0.0117

Mature and aged, male and female malate induced, complex I and succinate induced, complex II, active mitochondrial oxygen consumption (nM O2/min/mg mitochondrial protein) and RCI (state 3/state 4) for Control (after 75 minutes of perfusion), GI, K/Mg and K/Mg + DZX (after 30 minutes equilibrium, 30 minutes of ischemia. 15 minutes reperfusion). All results are shown as mean (SEM) for n = 7−8 animals for each group. The number of individual observations for each value is shown in parentheses. P values for specific comparisons between male and female animals, within an age group, were calculated using a t test. Significant differences at p < 0.05 are shown in bold face.

ATP = adenosine triphosphate; DZX = diazoxide; GI = global ischemia; K/Mg = potassium/magnesium.