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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Biochem Biophys Res Commun. 2008 May 20;372(3):412–417. doi: 10.1016/j.bbrc.2008.05.053

Table I.

Real time PCR analysis of EVA and E-cadherin expression from microdissected choroid plexus.

Condition EVA
(Ct ± SEM)
n= E-Cadherin
(Ct ± SEM)
n= GAPDH
(Ct)
Wild Type Untreated 26.81 ± 0.35* 9 26.24 ± 0.13* 6 20.0
Wild Type 2C11 27.38 ± 0.20 6 27.02 ± 0.19 6 20.0
RAG−/− Untreated 29.55 ± 0.13 9 29.18 ± 0.03 6 20.0
RAG−/− 2C11 28.18 ± 0.20** 6 27.88 ± 0.06* 6 20.0

Real time PCR analysis was performed as described in Materials and Methods. Ct values were normalized using GAPDH expression as a control (Ct=20.0). For EVA expression, *P<0.0001 and **P=0.0003 as compared to the RAG−/− untreated condition. For cadherin expression, *P<0.0001 for both conditions as compared to the RAG−/− untreated condition. Ct: Threshold cycle.