Table I.
Condition | EVA (Ct ± SEM) |
n= | E-Cadherin (Ct ± SEM) |
n= | GAPDH (Ct) |
---|---|---|---|---|---|
Wild Type Untreated | 26.81 ± 0.35* | 9 | 26.24 ± 0.13* | 6 | 20.0 |
Wild Type 2C11 | 27.38 ± 0.20 | 6 | 27.02 ± 0.19 | 6 | 20.0 |
RAG−/− Untreated | 29.55 ± 0.13 | 9 | 29.18 ± 0.03 | 6 | 20.0 |
RAG−/− 2C11 | 28.18 ± 0.20** | 6 | 27.88 ± 0.06* | 6 | 20.0 |
Real time PCR analysis was performed as described in Materials and Methods. Ct values were normalized using GAPDH expression as a control (Ct=20.0). For EVA expression, *P<0.0001 and **P=0.0003 as compared to the RAG−/− untreated condition. For cadherin expression, *P<0.0001 for both conditions as compared to the RAG−/− untreated condition. Ct: Threshold cycle.