Figure 3.

Characterization of the TSP-1-transfected SCL-I cells (SCL-I^TSP-1 clone 9). (A) Western blot analysis of control cells (vector clone A cells) and three SCL-I^TSP-1 clones (clones 3, 5, and 9). TSP-1 monomers from activated platelets (TSP-1) with a molecular mass of 180-kDa were used as standard. Expression of the 180-kDa TSP-1 protein is up-regulated in all three SCL-I^TSP-1 clones. (B) In vitro growth curves are similar for the parental SCL-I and SCL-I^TSP-1 clone 9 cells. (C) Tumor growth curve of SCL-I^TSP-1 clone 9 cells after s.c. injection of the cells in nude mice. In comparison with the control clone A cells (mean value of 10 animals), which developed large tumors in less than 60 days, SCL-I^TSP-1 cells are suppressed in their growth during this time span (10 individual animals).