Figure 5.

The tumor phenotype is reverted by antisense oligonucleotide treatment in vivo. (A) Histology of a 2-week-old tumor nodule of SCL-^TSP-1 clone 9 cells treated with antisense oligonucleotides. The originally cyst-like tumor has reverted to a carcinoma. (B) Double-labeling of a corresponding section with antibodies against TSP-1 (green; yellow when colocalized with blood vessels) and collagen type IV (red) showing few TSP-1 dots and extended tumor vascularization (arrow) in addition to blood vessels in the surrounding stroma (arrowhead). (C) SCL-I^TSP-1 cells treated with control oligonucleotides remain as cyst-like tumors with a prominent TSP-1 matrix (green) at the tumor/stroma border and blood vessels (red, arrowhead) restricted to the surrounding stroma. (Bar = 100 mm.)