Table 1.
Characterization of mosquito carboxypeptidases.
| Gene name | GenBank accession | Predicted protein length (aa) | Residue Length S/P/M | Specificity residue | Larval expression | Peak MG expression (hours PBM) | Rel. basal transcript level |
|---|---|---|---|---|---|---|---|
| Ae. aegypti | |||||||
| AaCPA-I | AY590487 | 427 | 21/92/314 | V | NE | 12-24 | 1.00 |
| AaCPA-II | AY590488 | 442 | 19/102/321 | V | Expressed | 12-36 | 0.77 |
| AaCPA-III | AY590489 | 421 | 18/90/313 | P | Expressed | 6-36 | 0.52 |
| AaCPA-IV | AY590490 | 414 | 19/88/307 | N | Expressed | NE | NE |
| AaCPA-V | AY590491 | 415 | 17/88/310 | R | Expressed | 36 | 8.77 |
| AaCPA-VI | AY590492 | 417 | 21/88/308 | I | Expressed | 36 | 9.10 |
| AaCPA-VII | AY590493 | 423 | 21/92/310 | M | NE | 3-24 | 10.63 |
| AaCPA-VIII | EAT39608 | 415 | 17/89/309 | V | Expressed | NE | NE |
| AaCPA-IX | EAT37218 | 422 | 21/92/309 | S | Expressed | NE | NE |
| AaCPA-X | EAT46817 | 414 | 22/84/308 | I | NE | NE | NE |
| AaCPA-XI | EAT44906 | 424 | 14/100/310 | I | Expressed | NE | NE |
| AaCPB-I | AY590494 | 412 | 18/89/305 | D | NE | 24-36 | 2.70 |
| AaCPB-II | AY590495 | 412 | 18/88/306 | D | Expressed | 3-6 | 2.32 |
| AaCPB-III | AY590496 | 425 | 20/93/312 | D | Expressed | 24-36 | 1.36 |
| AaCPB-IV | EF423586 | 437 | 20/93/324 | D | Expressed | 24 | 6.90 |
| AaCPB-V | EF423587 | 417 | 14/102/301 | D | NE | 3-36 | 0.08 |
| AaCPB-VI | EF423588 | 435 | 20/106/310 | D | NE | NE | NE |
| AaCPB-VII | EAT36173 | 457 | 19/129/309 | D | NE | NE | NE |
| Ae. polynesiensis | |||||||
| ApCPB-I | AY593983 | 412 | 18/89/305 | D | ND | ND | ND |
| Oc. triseriatus | |||||||
| OtCPB-I | AY593984 | 411 | 18/89/304 | D | ND | ND | ND |
| Oc. epactius | |||||||
| OeCPB-I | AY593985 | 411 | 18/89/304 | D | ND | ND | ND |
S: signal peptide, P: propeptide, M: mature peptide, NE: not expressed, ND: not determined,MG: midgut. Note that relative basal transcript levels were calculated from normalized levels of CPA-I transcripts in unfed mosquitoes using S7 ribosomal protein transcripts as the internal standard.