Fig. 2.

Production of prostaglandin D₂ (PGD₂), histamine and leukotriene C₄ (LTC₄) by mast cells. Mast cells were preincubated with immunoglobulin E (IgE) followed by treatment with culture medium or anti-IgE antibody in the presence or absence of diclofenac, as described in Materials and methods. One h after the treatments the supernatants were collected and assayed for PGD₂ (a), histamine (b) and LTC₄ (c) by enzyme immunoassay. Results are expressed as the mean ± standard error of the mean (n = 3–4). P < 0·0001 for (a) or P < 0·05 for (b,c) by analysis of variance. P > 0·05 by Newman–Keuls test for (a) no additive versus anti-IgE + 10 µM diclofenac, n = 4; (b) anti-IgE versus anti-IgE + 10 µM diclofenac, n = 3; (c) no additive versus anti-IgE, n = 3.