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. 2009 Apr;156(1):172–182. doi: 10.1111/j.1365-2249.2008.03872.x

Table 1.

Primer pairs for semiquantitative polymerase chain reaction.

Target gene Primer sequences Major product size (base pairs)
Mouse TNF-α Forward: 5′-TTCTGTCTACTGAACTTCGGGGTGATCGGTCC-3′ 354
Reverse: 5′-GTATGAGATAGCAAATCGGCTGACGGTGTGGG-3′
Human MDR1 Forward: 5′-AGATCAACTCGTAGGAGTGTC-3′ 795
Reverse: 5′-GTTTCTGTATGGTACCTGCAA-3′
Mouse β-actin Forward: 5′-GTGGGCCGCTCTAGGCACCAA-3′ 540
Reverse: 5′-CTCTTTGATGTCACGCACGATTTC-3′
Human β-actin Forward: 5′-GAGACCTTCAACACCCCAGCC-3′ 311
Reverse: 5′-GGCCATCTCTTGCTCGAAGTC-3′

MDR, multiple drug resistance; TNF, tumour necrosis factor.