Table 1.
Target gene | Primer sequences | Major product size (base pairs) |
---|---|---|
Mouse TNF-α | Forward: 5′-TTCTGTCTACTGAACTTCGGGGTGATCGGTCC-3′ | 354 |
Reverse: 5′-GTATGAGATAGCAAATCGGCTGACGGTGTGGG-3′ | ||
Human MDR1 | Forward: 5′-AGATCAACTCGTAGGAGTGTC-3′ | 795 |
Reverse: 5′-GTTTCTGTATGGTACCTGCAA-3′ | ||
Mouse β-actin | Forward: 5′-GTGGGCCGCTCTAGGCACCAA-3′ | 540 |
Reverse: 5′-CTCTTTGATGTCACGCACGATTTC-3′ | ||
Human β-actin | Forward: 5′-GAGACCTTCAACACCCCAGCC-3′ | 311 |
Reverse: 5′-GGCCATCTCTTGCTCGAAGTC-3′ |
MDR, multiple drug resistance; TNF, tumour necrosis factor.