Figure 4. Activation of the ADPR-dependent current in hippocampal slices in CA1 pyramidal neurons but not in stratum radiatum interneurons.

A, representative whole-cell recording from a CA1 pyramidal neuron demonstrating activation of an ADPR- dependent current (1 mm ADPR in the patch pipette). The amplitude of the current was reduced by extracellular application of 10 μm clotrimazole (CLT) as shown. Ramps were blanked for clarity. B, I–V curves from the same cell. Traces were taken from voltage-ramps applied at the start of recording (trace 1), after 30 min (trace 2) and following perfusion of the slice for 20 min with ACSF containing 10 μm CLT (trace 3). C, summary bar graph demonstrating inhibition of ADPR-dependent current by 10 μm CLT (n= 4). D, summary bar graph of ADPR-dependent currents induced by application of NMDA and abolished by removal of extracellular Ca²⁺ in CA1 pyramidal cells (n= 6 and 8) and stratum radiatum interneurons (n= 7 and 6).