Fig. 5.

Immunolocalization of CFTR in 35-day-old larvae (D35), transverse sections: (A,A’,B,D,E,H) SW-acclimated fish, (C,F,G,I) FW-acclimated fish. (A,A′,B) Crypt staining in ionocytes (I) of the gill filaments (F). (C) No staining in gills. (D) Basolateral staining in the posterior intestine (PI). (D–G) Weak diffuse staining in the collecting ducts. (E) Apical staining of the brush border in the rectum (R). (F) Weak apical staining in the posterior intestine (PI). (G) Apical staining of the brush border in the rectum (R). (H,I) Apical and diffuse staining in the urinary ducts (UD).