Immunolocalization of CFTR in 50-day-old larvae (D50), transverse sections: (A,C,E,G) SW-acclimated fish, (B,D,F,H) FW-acclimated fish. (A) Crypt staining in ionocytes (I) of gill filaments (F). (B) No staining in gills. (C,D–F) No staining in the urinary ducts (UD) and collecting ducts. (G) Apical and basal staining in the posterior intestine (PI). (H) Apical staining of the brush border in the posterior intestine (PI).