Competition assays in cell-free extracts containing increasing amounts of Alpha2. A. 50μl of in vitro produced Dab1 were incubated with 50 μl of in vitro produced Lis1 and different amount (0, 5, 10, 25, 50 μl) of in vitro produced Alpha2. Proteins were immunoprecipitated with Dab1 antibodies, detected by autoradiography, and normalized to the amount of precipitated Dab1. Histograms show the ratio of Lis1/Dab1 (Grey) and Alpha2/Dab1 (black). B. 50μl of in vitro produced VLDLR were incubated with 50 μl of Dab1 and different amount (0, 5, 10, 25, 50 μl) of Alpha2. Proteins were immunoprecipitated with VLDLR antibodies and normalized to the amount of precipitated receptor. Histograms show the ratio of Dab1/VLDLR (black) and Alpha2/VLDLR (grey).