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. Author manuscript; available in PMC: 2010 Apr 24.
Published in final edited form as: Brain Res. 2009 Mar 9;1267:1–8. doi: 10.1016/j.brainres.2009.02.059

Figure 4. Alpha2 competes with Lis1 for binding to Dab1 but does not competes with Dab1 for binding to VLDLR.

Figure 4

Competition assays in cell-free extracts containing increasing amounts of Alpha2. A. 50μl of in vitro produced Dab1 were incubated with 50 μl of in vitro produced Lis1 and different amount (0, 5, 10, 25, 50 μl) of in vitro produced Alpha2. Proteins were immunoprecipitated with Dab1 antibodies, detected by autoradiography, and normalized to the amount of precipitated Dab1. Histograms show the ratio of Lis1/Dab1 (Grey) and Alpha2/Dab1 (black). B. 50μl of in vitro produced VLDLR were incubated with 50 μl of Dab1 and different amount (0, 5, 10, 25, 50 μl) of Alpha2. Proteins were immunoprecipitated with VLDLR antibodies and normalized to the amount of precipitated receptor. Histograms show the ratio of Dab1/VLDLR (black) and Alpha2/VLDLR (grey).