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. 1999 Mar 2;96(5):2071–2076. doi: 10.1073/pnas.96.5.2071

Figure 2.

Figure 2

Interaction of SOCS-1 with endogenous elongins B and C. Cellular extracts from M1 cells stably expressing either N-terminally Flag-tagged full-length SOCS-1 or SOCS-1 lacking the SOCS box (SOCS-1/ΔSB) were incubated with anti-Flag antibody M2 resin and bound cellular proteins eluted with Flag peptide. Lanes 1–3 correspond to column eluates 3–5 from M1 cells expressing full-length SOCS-1, and lanes 4–6 correspond to column eluates 3–5 from M1 cells expressing SOCS-1/ΔSB. The panels from top to bottom correspond to Western blot analyses with anti-Flag, anti-elongin C, anti-elongin B, and a mixture of anti-elongin B and anti-elongin C, respectively.