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Interaction of full-length SOCS-1 with elongins B and C in 293T cells depends on cytokine stimulation and proteasomal inhibition to be visualized. Lysates from 293T cells cotransfected with Myc-elongin C and Flag-tagged elongin B and full-length SOCS-1 were immunoprecipitated with anti-Myc antibodies and Western blotted with anti-Flag antibodies (Top). Whole-cell lysate was Western blotted with anti-Flag or anti-Myc antibodies as loading controls (Middle and Bottom).
