FIG. 2.
Effect of MAbs a3-1d, d7-12c, a3-3a, and a3-7g on the induction of NO and TNF-α production by p40 family of cytokines in mouse peritoneal macrophages. Cells were stimulated with 10 ng/mL of p402, p40, IL-12, and IL-23 in the presence of different concentrations of purified MAbs a3-1d (A and E), d7-12c (B and F), a3-3a (C and G), and a3-7g (D and H) under serum-free condition. After 12 h of stimulation, supernatants were used for TNF-α assay (A–D), as described in the section on Materials and Methods. However, after 24 h of stimulation, supernatants were used for nitrite assay (E–H). Data are mean ± SD of three different experiments.