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Effect of MAbs a3-1d, d7-12c, a3-3a, and a3-7g on the induction of TNF-α production by p40 family of cytokines in mouse primary microglia. Cells were stimulated with 10 ng/mL of p402, p40, IL-12, and IL-23 in the presence of different concentrations of purified MAbs a3-1d (A), d7-12c (B), a3-3a (C), and a3-7g (D) under serum-free condition. After 12 h of stimulation, supernatants were used for TNF-α assay. Data are mean ± SD of three different experiments.
