FIG. 4.
Effect of MAbs a3-1d, d7-12c, a3-3a, and a3-7g on the induction of NF-κB activation in BV-2 microglial cells. Cells plated at 50–60% confluence in 12-well plates were cotransfected with 0.5 μg of pBIIX-Luc and 25 ng of pRL-TK. After 24 h of transfection, cells were stimulated with 10 ng/mL of p402, p40, IL-12, and IL-23 in the presence of different concentrations of purified MAbs a3-1d (A), d7-12c (B), a3-3a (C), and a3-7g (D) under serum-free condition. After 6 h of stimulation, firefly and Renilla luciferase activities were obtained by analyzing total cell extract, as described in the Materials and Methods section. Results are expressed as relative to control 1. Data are mean ± SD of three different experiments.