Figure 5. Decrease in hepatic hepcidin levels in intestine-specific Hif2a-knockout mice without any significant changes in duodenal FPN protein levels.

(A) Hepcidin mRNA expression in livers of vil-Cre⁺/HIF-1α^fl/fl and vil-Cre⁺/HIF-2α^fl/fl mice and WT littermates by real-time RT-PCR (n = 7). ***P < 0.001, unpaired Student’s t test. (B) FPN expression in duodenum scrapings of WT and vil-Cre⁺/HIF-2α^fl/fl mice from 3 independent littermates, by Western blotting. Results were quantified and normalized (FPN/α-tubulin).