Fig. 7.

Representative Western blot and densitometry of differential Triton extractability of Hsp70 from insoluble and soluble fractions from outer stripe of the outer medulla. Aliquots of Hsp-rich supernatant were incubated with cytoskeletal pellets isolated after low protein (RPs/LPi) or isolated from controls (RPs/NPi). Repeat Triton extraction was performed. Statistical analysis from three experiments confirmed the Hsp70 translocation into the injured cytoskeletal fraction (insoluble) after incubation of LPi in RPs (RPs/LPi), resulting in the appearance of Hsp70 Triton-insoluble signal higher than in the incubation of NPi in RPs (RPs/NPi) in the blot. Meanwhile, densitometric analysis showed a lower sol/insol Hsp70 ratio in RPs/LPi vs. RPs/NPi (***p < 0.001). Data are shown as mean ± SEM