Fig. 4.

Subcellular localization of sHsps. Tobacco protoplasts were transiently transformed for expression of the indicated myc-tagged sHsps. For mock transformation pRTNeo was used. sHsps were fluorescently labeled using myc-specific antibodies (red channel). a Intracellular distribution pattern of cytoplasmic/nuclear sHsp members. DAPI staining shows the localization of nuclei in the same cells (blue channel). b Organellar sHsp members. As a marker for mitochondrial localization, an expression construct encoding GFP fused to a mitochondrial targeting signal (mt-GFP) was cotransformed, and immunodetection of endogenous catalase (α-Cat) was used to visualize peroxisomes (green channel). Merged images illustrate sites of colocalization in yellow