Table 1.

Details on the investigated genes and the corresponding oligonucleotide primers used for qPCR

Gene ID	Gene symbol		Primer sequence	Exon junction	Product size (bp)	FlyBase ID
A	cg4269	F	5′-GAATGGCTAAGAATATGTTCA-3′	1–2	136	FBgn0034741
		R	5′-ACAGATTGCCGTGGTATTCGCTG-3′
B	cg6332	F	5′-TGCCCTTCGCTACAAGCCGAGT-3′	3–4	152	FBgn0038921
		R	5′-CGTGCTCGCCTTGTATTTGAGCG-3′
C	cg13227	F	5′-GAGAAGGAATACGACATCGAAATGGAT-3′	–	131	FBgn0033589
		R	5′-GAGCTTGGTTTCTCCTGCACCACT-3′
D	cg13664	F	5′-CGCCATTCTGGACTCACGTTGTT-3′	–	107	FBgn0039294
		R	5′-TGATCCGCAGCTTGCCGATTAT-3′
E	cg18331	F	5′-TCCACCACCTCATCTGACCCCG-3′	–	140	FBgn0036181
		R	5′-ACGACAGGAGCCGAAGAGGA-3′
F	cg32130	F	5′-GATGCTGACCCGCAACCTGCT-3′	3–4	106	FBgn0086708
		R	5′-ATCGAAGCCTGAATGACTTTGAT-3′
G	cg6734	F	5′-CTCATGTCCATTGCGGCTCTCTATG-3′	2–3	190	FBgn0032395
		R	5′-GTAGGTGCTCCATGATACTGGCGTC-3′
H	cg6766	F	5′-CGACAAGCCAGAGTTGTCTCCAATT-3′	2–3	189	FBgn0032398
		R	5′-TCATCGGTCCATTTGCCCAGGT-3′
I	cg6785	F	5′-TCTTTAGCTCCTAGCAGATCGCCTTA-3′	1–2	196	FBgn0032399
		R	5′-GATCCCAGATCTGCTTGAGGCTC-3′
J	cg6792	F	5′-CTTCAAGCTTAAGAACCCGCGAGCT-3′	1–2	194	FBgn0032401
		R	5′-GCCGTCGTAGGTGAGTGTCGTATTC-3′
K	cg14945	F	5′-CAAGGAGTTCCCAGTGGGTTTTGG-3′	1–2	215	FBgn0032402
		R	5′-CCACGGAACCAAACAGGACATCG-3′
–	Beta actin	F	5′-ATGACGCACCGCGTGCAGTT-3′	–	109	FBgn0000043
		R	5′-CTCTGTGCCTCATCGCCGACAT-3′

F Forward primer, R reverse primer