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. 2009 Feb 4;64A(1):34–44. doi: 10.1093/gerona/gln053

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© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Electrical field stimulation (EFS) evokes ryanodine-sensitive calcium responses in rat superior cervical ganglion (SCG) cells. Representative data in a single SCG cell from a (A) 6-month-old and (B) 24-month-old animal. Fifty pulses of EFS (5 Hz) were delivered at varied currents with a 2-minute equilibration separating each stimulation. Following the last stimulation, cells were exposed to Tyrode's buffer containing 100 μM of the ryanodine receptor antagonist ryanodine for 30 minutes, and the stimulation protocol was repeated (see Methods).