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. Author manuscript; available in PMC: 2009 Apr 27.
Published in final edited form as: Mol Endocrinol. 2006 Jun 13;20(11):2909–2930. doi: 10.1210/me.2006-0109

Fig. 1. Gene Expression Profiling in LβT2 Cells in Response to Activin.

Fig. 1

Cells were treated with 25 ng/ml activin or vehicle for 24 h. Total RNA was profiled on Affymetrix U74Av2 chips. A, Scatterplot of expression levels from activin-treated (y-axis) or basal cells (x-axis). Data are normalized using CORGON and log transformed. Parallel lines indicate 2-fold changes. Units are relative fluorescence. B, Validation of selected genes by Q-PCR. Graph shows fold change with activin treatment as measured by microarray (open bars) or Q-PCR (black bars). RNAs are from four independent experiments. C, Induction of gonadotropin and GnRH-receptor genes by activin by microarray (open bars) or Q-PCR (black bars). Gene symbols are consistent with National Center for Biotechnology Information Entrez Gene nomenclature. ND, Not detectable.