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. Author manuscript; available in PMC: 2009 Apr 27.
Published in final edited form as: Mol Endocrinol. 2006 Jun 13;20(11):2909–2930. doi: 10.1210/me.2006-0109

Fig. 4. Validation of Microarray Data by Q-PCR.

Fig. 4

Ten genes were chosen from the list of differentially regulated genes for validation on RNA from four independent experiments. Each Q-PCR measurement was made in triplicate. Data are standardized to the ATPase6d1 gene (vacuolar ATP synthase subunit d1 or physophilin). Left-hand panel for each gene shows the microarray results; right-hand panel shows the Q-PCR data. Black bars (■) denote activin-treated cells; open bars (□) denote untreated cells. Vertical axis shows relative fluorescence units; horizontal axis shows time of GnRH treatment.