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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Chem Res Toxicol. 2008 Sep 13;21(10):1983–1990. doi: 10.1021/tx800174a

Fig. 2.

Fig. 2

Replication bypass of DNA–peptide cross-links by human pol κ. Upper panel: Primer extensions by pol κ were carried out for 20 min in the presence of 100 µM dNTPs at 1.25 nM (lanes 3, 7, and 11), 2.5 nM (lanes 4, 8, and 12) or 5 nM (lanes 5, 9, and 13) protein concentrations. Lower panel: Single nucleotide incorporations by pol κ (1 nM) were carried out for 5 min in the presence of 20 µM individual dNTPs. M – oligodeoxynucleotide sizing markers; ND – non-damaged template; XL – cross-link-containing template.