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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Chem Res Toxicol. 2008 Sep 13;21(10):1983–1990. doi: 10.1021/tx800174a

Fig. 4.

Fig. 4

Replication bypass of γ-HOPdG, reduced γ-HOPdG, and DNA–peptide cross-links by E. coli pol IV. Upper panel: Primer extensions by pol IV were carried out for 20 min in the presence of 100 µM dNTPs at 1.25 nM (lanes 3, 7, 11, 15, and 19), 2.5 nM (lanes 4, 8, 12, 16, and 20) or 5 nM (lanes 5, 9, 13, 17, and 21) protein concentrations. Lower panel: Single nucleotide incorporations by pol IV (1 nM) were carried out for 20 min in the presence of 20 µM individual dNTPs. M – oligodeoxynucleotide sizing markers; ND – non-damaged template; XL – cross-link-containing template.