Figure 2. Equilibrium binding measurements of T4-Pdg and ds(2AP/TF).
Titrations were performed in PKC buffer using three fixed concentrations of ds(2AP/TF) corresponding to 10 nM (●), 20 nM (O), and 30 nM (θ). The lines drawn are from a global fit of the data described in the data analysis section. Shown is a representative data set with Kd = 24.9 ± 10.2 nM.
