. Author manuscript; available in PMC: 2009 Apr 27.

Published in final edited form as: Biochemistry. 2006 Nov 28;45(47):14192–14200. doi: 10.1021/bi060802s

Table 2.

Summary of kinetic and equilibrium constants derived for T4-Pdg with ds(2AP/TF) and ds(TAM-2AP/QSY-TF) substrates.

Substrate	Kd (nM) ^e	Kd (nM)^f	k_on (M⁻¹s⁻¹)^e	k_off (s^{− 1})^b,^e	k_{off (}s⁻¹)^b,^f
ds(2AP/TF)	28.4 ± 3.3^a	nd	9.78 × 10⁸ ± 0.2	24 ± 2.5^c	nd
ds(TAM-2AP/QSY-TF)	21.4 ± 4.2^a	26.7 ± 6.3^a	nd	25 ± 1^c	62 ± 5^d

: Mean ± SD n=3

: 6–9 replicate shots were collected and averaged per stopped-flow experiment.

: Mean ± SD n=4

: Mean ± SD n=5

: estimated from 2-AP fluorescence

: estimated from FRET analyses