Fig. 4.

Increased GRP78 expression with in vivo T cell activation and in vivo tolerance induction. KB5 synchimeric mice were untreated (left, n = 5), DST treated (middle, n = 4) to induce in vivo activation, or DST plus anti-CD154 mAb co-treated (right, n = 3) to achieve tolerance induction of transgenic DES⁺CD8⁺ T cells as described in the Materials and methods section. a Representative flow dot plots depicting CD8 expression (horizontal axis) and the anti-H2-K^b specific TCR recognized by the mAb DES (vertical axis) on lymphocytes obtained from the indicated treatment groups. DES⁺CD8⁺ T cells and DES⁻CD8⁺ lymphocyte populations are encircled. b Flow cytometric analyses of the activation marker CD44 on gated DES⁺CD8⁺ T cells (black line) and DES⁻CD8⁺ lymphocytes (shaded region). c Intracellular GRP78 expression in DES⁺CD8⁺ T cells (black line) and DES⁻CD8⁺ (shaded region) lymphocyte populations. The isotype control for GRP78 staining is indicated by a dotted line. d The mean of GRP78 protein expression displayed as bar graphs with error bars representing the SEM. Data are representative of four independent experiments