Table 2.

Primer and probe sequences for quantitative real time RT-PCR reactions

	Genes	Primer	Primer Sequence
1	CCT-alpha	F	5′-GCTTCTGGAGCAAGTATTCTGTCA-3′
		R	5′-TGTCCCAACATCACAGCTTCA-3′
		P	5′-6FAM-CCCTGGCCAACTTGGAAGGTG-TAMRA-3′
2	CCT-zeta	F	5′-ACGTGCTGCTGCATGAAATG-3′
		R	5′-TGAAGTAGTACCATCGCCAGTTATG-3′
		P	5′-6FAM-TTCAACACCCAACTGCCTCCTTAATAGCAA-TAMRA-3′
3	Rabbit GAPDH	F	5′-CGCCTGGAGAAAGCTGCTAA-3′
		R	5′-CCTCGGTGTAGCCCAGGAT-3′
		P	5′-6FAM-AAGCAGGCATCCGAGGGCCC-TAMRA-3′

The primers and probes were designed using Primer Express® software v2.0 provided by Applied Biosystems. Primer sequences are named as F and R, representing forward and reverse sequences respectively and P represents probe. Reverse primers (labeled R) are used in the RT reactions.