Single-cell culture analysis of O4+oligodendrocyte survival after 20 hours in the presence of pGEX-rEPF/cpn 10) and BV-EPF /cpn 10; comparison with other growth factors. All growth factors, (a) IGF-1, (b) PDGF, (c) bFGF, (d) insulin, (e) pGEX-rEPF and (f) BV-rEPF, promoted the survival of single-cell cultures of O4+ oligodendrocytes in a dose dependent manner. Data represent the mean percentage of viable cells after 20 hours of culture + SEM of four to five separate experiments. The significance of difference between means was measured using ANOVA with Tukey’s posttest