Table 1.
The percentage of GFP-expressing cells (above the gate at fluorescence intensity of 2000 in Figures 8 and 9).
Day | DNA onlya | LDH onlyb | 0.25 μg/mL DNA in LDH-DNAc | 0.50 μg/mL DNA in LDH-DNAc | 1.0 μg/mL DNA in LDH-DNAc |
---|---|---|---|---|---|
1 | 0.10 ± 0.05d | 0.41 ± 0.10 | 0.40 ± 0.30 | 0.77 ± 0.39 | 1.13 ± 0.58 |
2 | 0.04 ± 0.02 | 0.49 ± 0.19 | 0.84 ± 0.42 | 3.23 ± 1.66 | 3.95 ± 1.48 |
3 | 0.04 ± 0.01 | 1.35 ± 0.50 | 0.97 ± 0.21 | 2.18 ± 1.41 | 3.27 ± 1.45 |
Note: aThe plasmid DNA concentration was 1.0 μg/mL in the tests;
bThe LDH concentration was 100 μg/mL in the tests;
cThe plasmid DNA concentration was varied at 0.25, 0.50 and 1.0 μg/mL while the LDH concentration was kept at 25 μg/mL in the tests;
dThe average percentage and standard deviation were calculated from the data in 3 or 4 experiments.